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目的观察低频经颅磁刺激(LF-TMS)对匹罗卡品(PLO)致痫大鼠皮质脑电图及大鼠海马各区神经肽Y(NPY)表达的影响。方法雄性SD大鼠40只,随机分为对照组(PLO组)、干预组(PLO+LF- TMS组)。各组给予相关干预处理后腹腔注射PLO建立癫痫模型,观察皮质脑电图及海马HE染色、NPY免疫组化染色观察。结果两组间皮质脑电图指标比较,发现磁刺激干预后潜伏期延长、痫波频率及大发作次数降低。HE染色显示,对照组海马各区神经元显著变性、死亡,以海马CA3区明显;干预组海马各区神经元损害减轻。NPY的免疫组化染色显示对照组各时间点海马各区NPY阳性细胞增多,明显高于干预组,差异有统计学意义。结论LF-TMS不仅可以延迟PLO癫痫点燃的形成,还可以缓解点燃后的癫痫发作程度;LF-TMS可明显影响海马各区NPY阳性细胞数。NPY与癫痫发作程度相关,提示NPY表达与癫痫之间关系紧密,NPY可能具有抗痫和神经保护作用。
Objective To observe the effects of low-frequency transcranial magnetic stimulation (LF-TMS) on cortical electroencephalogram and neuropeptide Y (NPY) expression in hippocampus of epileptic rats induced by pilocarpine (PLO). Methods Forty male Sprague-Dawley rats were randomly divided into control group (PLO group) and intervention group (PLO + LF-TMS group). The rats in each group were injected intraperitoneally with PLO to establish the model of epilepsy. The cortical EEG and hippocampal HE staining and NPY immunohistochemical staining were observed. Results Compared with the indexes of cortical EEG in the two groups, it was found that the latency of magnetic stimulation prolonged, the frequency of epileptic waves and the number of major seizures decreased. HE staining showed that the neurons in the hippocampus of the control group were significantly degenerated and died, and the hippocampal CA3 region was markedly impaired. The damage of neurons in the hippocampus of the intervention group was alleviated. Immunohistochemical staining of NPY showed that the number of NPY positive cells in hippocampus in control group increased significantly at each time point, which was significantly higher than that in intervention group. The difference was statistically significant. Conclusion LF-TMS can not only delay the formation of PLO epilepsy, but also alleviate the degree of seizures after ignition. LF-TMS can significantly affect the number of NPY positive cells in hippocampus. NPY and the degree of seizure related, suggesting that the relationship between NPY expression and epilepsy, NPY may have anti-epileptic and neuroprotective effects.