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溶组织内阿米巴的敏感株和抗性株均可激活补体,但抗性株可逃避膜攻击复合物的杀伤作用。本文目的在于确定溶组织内阿米巴滋养体耐受人血清溶解作用的机制。 溶组织内阿米巴滋养体HMI:IMSS株培养于36.5℃、无菌的TYI-S-33培养基中,阿米巴抗人血清溶解实验按Calderon和Tovar(1986)的方法进行。用经酵母多糖灭活补体的血清做对照。分析溶解作用时,溶解百分比为(对照组中活细胞数—血清组中活
Enzymes and resistant strains of Entamoeba histolytica can activate complement, but resistant strains can avoid the killing effect of membrane attack complex. The purpose of this article is to determine the mechanism of serolytic tolerance of Entamoeba histolytica trophozoites in humans. E. histolytica trophozoites HMI: The IMSS strain was cultured in sterile TYI-S-33 medium at 36.5 ° C. The amoeba anti-human serum lysis assay was performed as described by Calderon and Tovar (1986). Serum that was used to inactivate complement by zymosan was used as a control. Dissolution was analyzed as percent of viable cells in the control group - live in the serum group