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目的:应用光谱测量局部组织的水肿和血流动力学变化,评价光学光谱在不同人群中对牙周组织多种炎性指标(组织的氧合作用、组织的总血红蛋白量、脱氧血红蛋白、含氧血红蛋白和组织水肿)的评估能力。方法:使用手提式的近红外光谱测量仪分别从健康组织区域(n=54)、牙龈炎区域(n=50)、牙周炎区域(n=26)获取光谱并对其进行处理及评估。使用非参数的散射损失函数的改良比尔-兰伯特分解方式来研究每一种炎性组分在整体光谱中的相对分布。结果:光学光谱成功地用于评估牙周组织中复杂的炎症环境。数字表明牙周炎组织的氧合作用与牙龈炎区域及健康组织区相比明显降低(P<0.05)。这主要是由于在牙周炎区域的脱氧血红蛋白含量比牙龈炎区域(P=0.05)及健康组织区(P<0.01)明显增加所造成的。组织的含水量没有统计学差异,但是当牙周炎区域与牙龈炎区域及健康组织区域相比时,水指数相关的组织电解质水平及温度仍有统计学差异(P<0.03)。结论:本项研究进一步证实光学光谱能够在人类牙周组织中直接测量炎性指标。近红外线可见光谱可作为一种简单的、低耗的、无创伤、椅旁的、定位精确的诊断工具用于牙周病的诊断。
OBJECTIVE: To measure the changes of edema and hemodynamics of the local tissues by using optical spectroscopy, and to evaluate the effects of optical spectroscopy on various inflammatory parameters (tissue oxygenation, tissue total hemoglobin, deoxyhemoglobin, oxygen Hemoglobin and Tissue Edema). Methods: Spectra were obtained from the healthy tissue area (n = 54), gingivitis area (n = 50), periodontitis area (n = 26) and processed and evaluated using a hand-held near infrared spectrometer. The modified Beer-Lambertian decomposition of the nonparametric scattering loss function was used to study the relative distribution of each of the inflammatory components in the overall spectrum. Results: Optical spectroscopy was successfully used to assess the complex inflammatory environment in periodontal tissues. Figures show that periodontitis tissue oxygenation and gingivitis area and healthy tissue area was significantly lower (P <0.05). This is mainly due to a significant increase in deoxyhemoglobin content in the periodontitis area compared to the gingivitis area (P = 0.05) and the healthy tissue area (P <0.01). Tissue water content was not statistically different, but there was still a statistically significant difference (P <0.03) in tissue electrolyte level and temperature associated with water index when periodontal areas were compared with gingivitis areas and healthy tissue areas. Conclusion: This study further confirms that optical spectroscopy can directly measure inflammatory markers in human periodontal tissues. Near-infrared visible spectroscopy can be used as a simple, low-cost, non-invasive, chair-side, precisely-located diagnostic tool for the diagnosis of periodontal disease.