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目的通过比较手足口病患儿咽拭子和粪便标本病原阳性检出率,探究不同类型标本对手足口病实验室诊断的指导意义。方法 2012年2-11月份,收集全市7个县手足口病患儿咽拭子和粪便标本开展病原学检测,通过SPSS和Excel软件,对检测结果进行统计学分析。结果随机抽取手足口病患儿共281例,配对采集病例两种类型标本共562份,咽拭子、粪便的总肠道病毒阳性检出率分别56.58%、80.78%,EV71阳性检出率分别为17.79%、26.69%,CoxA16阳性检出率分别为3.56%、5.69%,两种类型标本的总肠道病毒阳性检出率(P<0.001)、EV71阳性检出率(P<0.001)、CoxA16阳性检出率(P=0.031)差异均有统计学意义。结论在手足口病实验室诊断中,粪便标本病原阳性检出率比咽拭子高,在日后工作中为提高标本阳性检出率,应首先选择采集粪便标本加以检测。
Objective To compare the positive detection rate of throat swabs and stool specimens in children with hand-foot-mouth disease and explore the significance of different types of specimens in laboratory diagnosis of hand-foot-mouth disease. Methods From Jan. to Nov. 2012, throat swabs and stool specimens of children with hand-foot-mouth disease in 7 counties of the city were collected for pathogenic detection. SPSS and Excel software were used for statistical analysis of the test results. Results A total of 281 children with hand-foot-mouth disease were randomized and 562 cases were collected from two types of matched samples. The positive rates of total enterovirus in throat swab and feces were 56.58% and 80.78% (17.79%, 26.69%). The positive rates of CoxA16 were 3.56% and 5.69% respectively. The positive rates of total enterovirus (P <0.001) and EV71 (P <0.001) CoxA16 positive detection rate (P = 0.031) were statistically significant differences. Conclusions In the laboratory diagnosis of hand-foot-mouth disease, the positive detection rate of stool specimens in stool specimens is higher than that in throat swabs. In order to improve the positive detection rate of stool specimens in future, stool specimens should be collected for detection.