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利用某些微生物代谢过程中产生酶的特点 ,筛选出能够大量生成葡萄糖氧化酶的地衣芽孢杆菌 ,并将其固定在Sephadex 10 0或海藻酸纳 -氯化钙 (载体 )上 ,首次研制成微生物酶传感器 .它不仅具有酶传感器的所有优点 ,同时也克服了酶传感器的缺点 ,使用寿命长 ,性质稳定 ,成本低 ,易于保存等 .通过优化微生物和其代谢所产生的葡萄糖氧化酶的固定化方法和利用葡萄糖氧化酶氧化葡萄糖产生过氧化氢的反应机理 ,建立了模拟酶 (hemin)催化荧光底物N ,N′ 二腈甲基邻苯二氨 (DCM OPA)检测体液中葡萄糖含量的荧光传感器 .
Bacillus licheniformis, which can generate glucose oxidase in large quantities, was screened out based on the characteristics of enzymes produced during the metabolism of some microorganisms and immobilized on Sephadex 10 0 or sodium-calcium alginate (carrier) It not only has all the advantages of enzyme sensor, but also overcomes the shortcomings of enzyme sensor, long service life, stable property, low cost, easy to save etc. By optimizing the immobilization of glucose oxidase produced by microorganism and its metabolism Method and the reaction mechanism of using glucose oxidase to oxidize glucose to produce hydrogen peroxide, a fluorescence spectrophotometry was developed to detect the fluorescence of glucose in body fluid by using hemin-catalyzed fluorescent substrate N, N’-dinitrotolylphthalide (DCM OPA) Sensor.