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〔目的〕通过实验查明造成此次疫情流行的病原菌 ,分析影响检验结果的各种原因。〔方法〕采用血、骨髓、粪便病原菌培养方法、生化反应、血清凝集实验、肥达氏反应、抗原酶联免疫实验方法和药物敏感性试验。〔结果〕由于患者血、骨髓、粪便中培养出伤寒杆菌 ,且经电子显微镜检测和L型细菌培养证实该流行菌株发生了L型变异 ,证明此疫情为L型伤寒流行 ,但疫区水源中未检出病原菌 ,肥达氏反应和酶联免疫抗原检测阳性率较低。〔结论〕本次流行菌株对青霉素、头孢哌酮等抗菌素耐药。须高度重视L型伤寒菌株的实验室检测。
[Objective] To identify the pathogens causing the epidemic through experiments and analyze the various causes that affect the test results. [Methods] Blood, bone marrow, fecal pathogen culture method, biochemical reaction, serum agglutination test, Widal reaction, antigen ELISA and drug sensitivity test were used. [Results] Salmonella typhimurium was cultured in the blood, bone marrow and feces of the patients and the L-type variation of the strain was confirmed by electron microscopy and L-type bacterial culture. This confirmed the epidemic was typhoid L epidemic, No pathogen was detected, the positive rate of Widal reaction and enzyme-linked immunosorbent assay was lower. 〔Conclusion〕 The epidemic strains of penicillin, cefoperazone and other antibiotics resistant. Laboratory testing of L-type typhoid strains should be given high priority.