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目的 探讨非小细胞肺癌 (NSCLC)组织内微血管密度 (MVD)和微血管结构 (MVS)特征与患者预后的关系。方法 采用免疫组化LsAB法 ,以Von Willebrand因子抗体显示血管内皮细胞 ,镜下观察 49例NSCLC患者肿瘤组织中微血管的数量与结构特征。根据其形态的不同 ,把微血管分为A、B两型 ,A型主要表现为血管壁较厚或较完整 ,管形较规则 ,常分散存在 ;B型主要表现为血管壁薄或不完整 ,管形不规则 ,常丛状或网状分布。结果 ①原发NSCLC组织内MVD与患者pTNM分期和淋巴结受累与否密切相关 ,P值分别为0 .0 43和 0 .0 3 8,而MVS与原发瘤大小密切相关 ,P =0 .0 0 2 ;②原发NSCLC组织内MVD较高者 (>5 2 /2 0 0×视野)的生存期 (2 3 .2月± 18.4月 )明显短于MVD较低者 (<5 2 /2 0 0×视野) (3 5 .9月± 2 0 .9月 ) ,P =0 .0 1;③原发NSCLC组织内MVSA型者的生存期 (3 9.4月± 17.2月 )明显长于MVSB型者 (2 3 .5月± 2 0 .3月 ) ,P =0 .0 0 8;④原发NSCLC组织内MVD <5 2 /2 0 0×视野 且MVSA型者的生存期 (4 2 .9月± 19.3月 )明显长于MVD >5 2 /2 0 0×视野 且MVSB型者 (15 .7月± 16.8月 ) ,P =0 .0 0 2。结论 原发NSCLC组织内MVD和MVS特征与患者预后密切相关 ,提示MVD和MVS特征可以作为临床估价患者预后和设计术后辅助治
Objective To investigate the relationship between the characteristics of microvessel density (MVD) and microvascular structure (MVS) in non-small cell lung cancer (NSCLC) and the prognosis of patients. Methods Using immunohistochemical method of LsAB, Von Willebrand factor antibody was used to detect vascular endothelial cells. The number and structure of microvessels in 49 NSCLC patients were observed microscopically. According to their morphological differences, the microvessels are divided into A, B two types, A type is mainly manifested as thicker or more complete blood vessel wall, the tube is more regular, often dispersed; B-type is mainly manifested as thin or incomplete vascular wall, Tubular irregular, often plexiform or reticular distribution. Results ① The MVD in primary NSCLC was closely related to the pTNM stage and lymph node involvement in patients with primary NSCLC, with P values of 0.043 and 0.308, respectively. The MVS was closely related to the size of primary tumor (P = 0.0) 0 2; ② The survival of patients with higher MVD (> 52/200 X field) in primary NSCLC was significantly shorter than that of lower MVD (<5.2% 0 0 × field of view) (3.5 ± 2.0 months, September = 0.9), ③ The survival of MVSA type in primary NSCLC tissues was significantly longer than that of MVSB type (39.9 ± 17.2 months) (23.5 months ± 2.0.3 months), P = 0.080; ④The survival of patients with MVD <52/200 × visual field and MVSA type in primary NSCLC tissues (42). September ± 19.3 months) was significantly longer than MVD> 52/200 × field of vision and MVSB type (15.7 ± 16.8 months), P = 0.002. Conclusion The characteristics of MVD and MVS in primary NSCLC tissues are closely related to the prognosis of patients, suggesting that the characteristics of MVD and MVS can be used as clinical evaluation of prognosis and postoperative adjuvant therapy