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目的研究珠芽蓼果实粉的急性毒性和遗传毒性,为珠芽蓼果实粉的进一步开发利用提供安全依据。方法按照GB 151930—2003《食品安全性毒理学评价程序和方法》,进行急性经口毒性试验、Ames试验、小鼠骨髓嗜多染红细胞微核试验及小鼠精子畸形试验。结果急性经口毒性试验中实验组动物7 d内未见中毒症状,最大耐受剂量(MTD)>7.50 g/kg体重。Ames试验中各组回变菌落数均未超过自发回变菌落数2倍,且无剂量反应关系,结果为阴性。骨髓微核试验中各剂量组雌雄性别微核形成率与阴性对照结果比较,差异无统计学意义(P>0.05),结果为阴性。小鼠精子畸形试验中各实验组与阴性对照组结果比较,差异无统计学意义(P>0.05),精子畸形试验结果为阴性。结论本次试验条件下,珠芽蓼果实粉未显示有急性毒性和遗传毒性作用。
Objective To study the acute toxicity and genotoxicity of Polygonum viviparum fruit powder and provide a safety basis for the further development and utilization of Polygonum viviparum fruit powder. Methods Acute oral toxicity test, Ames test, mouse bone marrow polychromatic erythrocyte micronucleus test and mouse sperm abnormality test were conducted according to GB 151930-2003 Food Safety Toxicology Evaluation Procedure and Method. Results In the acute oral toxicity test, the animals in the experimental group showed no symptoms of poisoning within 7 days and the maximum tolerated dose (MTD) was> 7.50 g / kg body weight. In the Ames test, the number of colonies returned by each group did not exceed 2 times the number of spontaneous return colonies, and no dose-response relationship, the result was negative. In the bone marrow micronucleus test, there was no significant difference between the male and female micronuclei in each dose group and the negative control group (P> 0.05). The result was negative. There was no significant difference between the experimental group and the negative control group in the sperm deformity test (P> 0.05). The sperm deformity test result was negative. Conclusion Under the test conditions, the powder of Polygonum viviparum fruits did not show acute toxicity and genotoxicity.