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目的 检测眠尔康胶囊对鼠伤寒沙门氏菌是否具有致突变作用。方法:按Ames提出的方法进行。采用掺入法和点试法,在两种方法中均分别加或不加体外活化系统(S9混合液)对受试物进行测试。共设5个剂量组,剂量设计分别为0.001、0.005、0.05、0.5、5mg/皿,另设阴性对照(自发回变)、阳性物溶剂对照组和阳性对照组,每个剂量做三块平皿,试验重复一次。结果:眠尔康胶囊Ames试验掺入法和点试法结果表明,在有无体外活化系统(S9)存在的情况下,均为阴性结果。结论:眠尔康胶囊对鼠伤寒沙门氏菌无致突变作用。
Objective To investigate whether Minglek capsule has a mutagenic effect on Salmonella typhimurium. Method: According to the method proposed by Ames. The test substance was tested by both the incorporation method and the spot test method with or without the activation system (S9 mixture) in both methods. A total of five dose groups, dose design were 0.001,0.005,0.05,0.5,5 mg / dish, another set of negative control (spontaneous back change), the positive control group and positive control solvent, each dose to do three plates The test is repeated. Results: Ames test incorporation and spot test results show that with or without activation system (S9) in the presence of the case, were negative results. Conclusion: Miankang capsule has no mutagenic effect on Salmonella typhimurium.