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目的:对三九胃泰方抗胃溃疡的有效部位进行筛选。方法:将SD大鼠分为正常对照组、模型组、阳性对照(西米替丁(0.1 g.kg-1)与三九胃泰颗粒剂(2 g.kg-1)组、石油醚萃取物组、醋酸乙酯萃取物组、正丁醇萃取组及水萃取组,按生药剂量均为2 g.kg-1ig给药7 d,采用乙醇造大鼠胃黏膜损伤模型,以胃溃疡抑制率、胃蛋白酶活力、血清中丙二醛(MDA)含量与超氧化物歧化酶(SOD)活力为检测指标,对不同极性溶剂(石油醚、醋酸乙酯、正丁醇与水)萃取部位的抗胃溃疡活性进行评价。结果:三九胃泰方各极性萃取部位均能显著抑制乙醇致大鼠胃黏膜损伤(P<0.05),其中石油醚萃取部位胃溃疡抑制率最高(89.15%),高于西米替丁组(59.90%)及三九胃泰颗粒剂组(55.31%)。三九胃泰方各极性萃取部位均可显著降低大鼠胃蛋白酶活力,同时增强血清中SOD的活力(P<0.05)。结论:三九胃泰方不同极性萃取部位均具有显著的抗胃溃疡作用,其中石油醚萃取部位活性最强。
Objective: To screen the effective site of Sanjiuweitaifang against gastric ulcer. Methods: The SD rats were divided into normal control group, model group, positive control (cimetidine 0.1 g.kg-1) and Sanjiuweitai Granules (2 g.kg-1) Ethyl acetate extract group, n-butanol extraction group and water extraction group, according to the crude drug dose of 2 g.kg-1ig administered for 7 days, the model of gastric mucosa injury was induced by ethanol and the inhibition of gastric ulcer , Pepsin activity, serum malondialdehyde (MDA) content and superoxide dismutase (SOD) activity as the detection indexes. The extraction of different polar solvents (petroleum ether, ethyl acetate, n-butanol and water) (P <0.05). The inhibition rate of peptic ulcer in petroleum ether extraction site was the highest (89.15% ) Was higher than that of cimetidine group (59.90%) and Sanjiuweitai Granules group (55.31%) .Solar extracts of Sanjiuweitaifang could significantly reduce pepsin activity in rats and enhance serum SOD activity (P <0.05) .Conclusion: Sanjiuweitai Fang has significant anti-gastric ulcer effect in different polar extraction sites, among which petroleum ether extraction site Strongest.