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目的 :探讨金环蛇毒心脏毒对 S180 ,EAC腹水癌细胞的细胞毒作用。方法 :采用小白鼠腹腔和皮下接种 S180 ,EAC腹水癌细胞造成小白鼠腹水模型后腹腔注射金环蛇毒心脏毒。结果 :腹腔注射金环蛇毒心脏毒 ,能抑制肿瘤细胞的生长 ,降低接种率。但不能完全控制腹水和癌细胞的生长。体外试验表明有明显的细胞毒作用。台酚蓝染色镜检可见死细胞显著增加 ,腹水图片检查 ,给药后细胞膜破裂 ,纤维化坏死明显。结论 :能延长小白鼠存活时间
Objective : To investigate the cytotoxic effects of the heart venom of Rhodobacter sinensis on S180 and EAC ascites carcinoma cells. METHODS : The mice were vaccinated intraperitoneally and subcutaneously with S180 , and EAC ascites cancer cells caused ascites model in mice after intraperitoneal injection of Golden Ring Snake Venom. RESULTS: Intraperitoneal injection of B. venom in the heart can inhibit the growth of tumor cells and reduce the inoculation rate. However, the growth of ascites and cancer cells cannot be completely controlled. In vitro tests showed a clear cytotoxic effect. The chlorophenol blue staining showed that the number of dead cells was significantly increased. Ascites were examined. After the administration, the cell membrane was ruptured and the fibrosis and necrosis were obvious. Conclusion :It can prolong the survival time of mice