应用大鼠经口、经皮肤亚慢性毒性试验 ,对海洋低温碱性蛋白酶毒理学进行研究。 12 0只Wistar大鼠 ,随机分为 6组 :对照组 ,海洋低温碱性蛋白酶 1.2 g/ kg(经口 ) ,1.2 g/ kg、0 .135g/ kg、0 .0 15g/ kg(经皮 )和 1.2 g/ kg(经皮追踪组 ) ,用药周期 90 d,追踪组增加 2 8d观察。记录和检测大鼠的一般状况和体质量增长、脏器系数、血常规、凝血时间、肝肾功、部分血液生化和离子浓度及病理检查。结果表明 ,海洋低温碱性蛋白酶各组 (经皮、经口 )大鼠的各项指标与对照组比较均未见明显异常 ( P均 >0 .0 5) ,提示大鼠能耐受长期给予 1.2 g/ kg(经皮、经口 )的海洋低温碱性蛋白酶 The rat oral and dermal subchronic toxicity test was used to study the low temperature alkaline protease toxicology in the sea. 120 Wistar rats were randomly divided into 6 groups: the control group, marine low-temperature alkaline protease 1.2 g / kg (oral), 1.2 g / kg, 0.135 g / kg and 0.15 g / kg ) And 1.2 g / kg (transdermal tracing group). The medication period was 90 days and the tracing group increased by 28 days. The general condition and body weight gain, organ coefficient, blood routine, clotting time, liver and kidney function, partial blood biochemistry and ion concentration and pathological examination were recorded and tested. The results showed that there was no obvious abnormality in each index of marine low temperature alkaline protease (percutaneous, oral) rats compared with the control group (all P> 0.05), suggesting that rats can tolerate long-term 1.2 g / kg (transdermal, oral) marine low temperature alkaline protease