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目的探讨诱导人骨髓间充质细胞在大鼠肝损伤模型中转分化为肝样细胞的可能性。方法建立肝叶切除和亚致死量照射两种肝脏损伤模型,植入人间充质干细胞,检测大鼠谷丙转氨酶、总胆红素和凝血酶原时间的变化;聚合酶链反应检测大鼠肝脏内人特异性DNA序列Alu-sx。结果造模后行干细胞移植组大鼠肝功能均有明显改善。聚合酶链反应显示各干细胞移植组大鼠肝脏中均能同时检测人特异性DNA序列Alu-sx和大鼠特异性DNA序列Sox11。结论人骨髓间充质干细胞能在肝损伤大鼠肝脏内存活,表达人特异性抗原,并能在肝损伤大鼠肝脏内分化为具有肝功能的肝样细胞,有助于肝损伤大鼠肝功能恢复。
Objective To investigate the possibility of inducing human bone marrow mesenchymal cells to transdifferentiate into hepatocyte-like cells in a rat model of liver injury. Methods Two models of hepatic injury induced by hepatectomy and sublethal irradiation were established. Human mesenchymal stem cells (MSCs) were implanted into the liver to detect the change of alanine aminotransferase, total bilirubin and prothrombin in rats. The liver of rat was detected by polymerase chain reaction Human-specific DNA sequence Alu-sx. Results After transplantation, the hepatic function of the stem cell transplantation group was significantly improved. Polymerase chain reaction showed that both human-specific DNA sequence Alu-sx and rat-specific DNA sequence Sox11 were simultaneously detected in the liver of each stem cell transplantation group. Conclusion Human bone marrow mesenchymal stem cells can survive in the livers of rats with liver injury and express human specific antigens, and can differentiate into liver-like hepatocytes in the liver of rats with liver injury and contribute to liver injury in rat liver Function recovery.