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将“津引薯 8号”马铃薯薄片和含有CaMV35S启动子控制的类甜蛋白 (TLP)基因与紧密连锁的Ubiquitin启动子控制的bar标记基因的农杆菌双元表达载体进行共培养。切下马铃薯薄片表面产生的幼芽 ,用含有除草剂bialaphos的MS培养基进行初步筛选。当获得的生根抗性芽生长成株后用TLP蛋白抗体和TLP基因特异引物对成株进行间接ELISA检测和PCR检测。两种检测结果一致率为 90 % ,推断以bar基因为筛选标记、紧密连锁的外源类甜蛋白基因已经进入马铃薯基因组并得以表达
The “Jinyatsu 8” potato flakes and the Agrobacterium binary expression vector containing the CaMV35S promoter-controlled taurin (TLP) gene and the closely linked Ubiquitin promoter-controlled bar marker gene were co-cultured. Shoots produced on the surface of potato slices were cut and initially screened in MS medium containing the herbicide bialaphos. When the obtained rooting-resistant buds grew into adult plants, the strains were subjected to indirect ELISA and PCR assays using TLP antibody and TLP gene-specific primers. The concordance rate of the two test results was 90%. The bar gene was deduced to be a screening marker, and the closely linked exogenous thaumatin gene had entered the potato genome and was expressed