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目的通过对部分卵胞浆内单精子显微注射(Half-ICSI)与短时受精结合早期补救ICSI两种授精方式卵子利用率、胚胎质量、妊娠结局及体外受精(IVF)部分受精失败率的比较,探寻临界精液质量患者最适合的授精方式。方法选取2012年4月-2014年6月在该院生殖中心第1周期获卵≥8枚并且配偶为临界精液质量患者49例,根据授精方式不同随机分为部分ICSI组(Half组,25例)和短时受精组(短时组,24例)。比较两组受精、卵裂情况,胚胎质量,着床率、妊娠率、成熟卵子利用率,授精方式为IVF的成熟卵子利用率及IVF部分的完全受精失败(TFF)率及低受精(LFR)率的差异。结果 Half组IVF部分的双原核(2PN)受精率及授精方式为IVF的成熟卵子利用率分别为44.17%和22.30%,高于短时组的15.46%和14.83%。Half组IVF部分LFR率(12.00%)低于短时组(62.50%)。结论对于临界精液质量患者,应用部分ICSI与短时受精结合早期补救ICSI的授精方式均能有效避免受精失败的发生,两者间胚胎质量及临床结局无差异,但前者可获得更多的授精方式为IVF的可利用胚胎。
Objective To investigate the effects of partial fertilization on egg utilization, embryo quality, pregnancy outcome and partial in vitro fertilization (IVF) fertilization rate by using partial sperm injection of half-ICSI and short-term fertilization combined with ICSI Compare, explore critical sperm quality in patients with the most appropriate way of insemination. Methods From April 2012 to June 2014, 49 patients with ovulation≥8 in the first cycle of the reproductive center and spouse of the spouse as the critical semen quality were selected and randomly divided into partial ICSI group (25 cases in the half group, ) And short-term fertilization group (short-term group, 24 cases). Fetal fertility, cleavage, embryo quality, implantation rate, pregnancy rate, mature egg utilization rate, fertilized IVF fertilization rate and complete fertilization failure (IVF) rate and IVF fertilization (LFR) Rate difference. Results The rate of fertilization and IVF fertilization of IVF in Half Group IVF was 44.17% and 22.30%, respectively, higher than 15.46% and 14.83% in short-term group. Half group IVF partial LFR rate (12.00%) was lower than the short-term group (62.50%). Conclusion For the patients with critical semen quality, the application of partial ICSI and short-term fertilization combined with ICSI early remediation methods can effectively prevent fertilization failure, there is no difference in embryo quality and clinical outcome between the two, but the former can get more insemination Available embryos for IVF.