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目的探讨人参胡核汤对人前列腺癌PC-3细胞的增殖抑制作用。方法通过血清药理学的方法,制备人参胡核汤高、中、低剂量及空白组的血清。各组与PC-3细胞作用48h、72h后,光镜观察细胞形态学改变;MTT法观察细胞增殖的抑制率;RT-PCR技术检测前列腺特异性抗原(PSA)表达。结果人参胡核汤含药血清各组作用48h、72h后,表现出不同程度的细胞皱缩,边缘毛糙,细胞核固缩或碎裂,细胞体积缩小;各组均可抑制PC-3细胞增殖;降低PSA表达水平。上述指标中,以人参胡核汤含药血清高剂量组作用48h的效果最为明显。结论人参胡核汤含药血清可使PC-3细胞的形态发生改变、抑制PC-3细胞增殖、降低PSA的表达。
Objective To investigate the inhibitory effect of Hugan Decoction on the proliferation of human prostate cancer PC-3 cells. Methods Serum of ginseng Hugan Decoction high, medium and low dose and blank group were prepared by serum pharmacology method. The cells were treated with PC-3 for 48h and 72h respectively. Morphological changes were observed with light microscope. The inhibition rate of cell proliferation was observed by MTT assay. The expression of prostate-specific antigen (PSA) was detected by RT-PCR. Results The serum of Hugan decoction group showed inhibitory effects on the proliferation of PC-3 cells after treated for 48h and 72h with different degrees of cell shrinkage, rough edges, pyknotic or fragmented nuclei, Reduce PSA expression level. Among the above indicators, the effect of the high dose of ginseng Hu nuclear decoction containing serum for 48h was the most obvious. Conclusions Hugan decoction containing ginseng can change the morphology of PC-3 cells, inhibit the proliferation of PC-3 cells and decrease the expression of PSA.