论文部分内容阅读
将小粒野生稻 (Oryzaminuta)、高粱的DNA分别导入水稻保持系V2 0B和IR5 80 2 5B ,在第 1代 (D1 )获得变异 ,从前者变异株的后代中选育出了新的不育系及其保持系株系野威A和野威B ,而高粱DNA导入IR5 80 2 5B获得的变异系从D2 开始在形态上已不出现分离。SSR分析表明 ,变异系野威B、香粱 5均与受体存在遗传多态性 ,并含有供体特异的分子标记带型。首次从分子水平上证明 ,通过导入外源DNA获得的变异株确实存在无分离的现象。说明导入远缘物种DNA是创造水稻新种质的有效途径。
The Oryzaminuta and sorghum DNAs were introduced into the rice maintainer lines V2 0B and IR5 80 2 5B, respectively, and the first generation (D1) was mutated. The new sterile lines were selected from the progenies of the former mutant And its maintainer lines, Weiwei A and Weiwei B, while the variation of sorghum DNA introduced into IR5 80 2 5B did not appear morphologically segregated from D2. SSR analysis showed that the variation lines Wild Granville B and Sweet Sorghum 5 both had genetic polymorphisms with the receptor and contained donor-specific molecular marker bands. For the first time, it was demonstrated from the molecular level that there is indeed no segregation of the mutants obtained by introducing exogenous DNA. This shows that the introduction of distant species DNA is an effective way to create new rice germplasm.