论文部分内容阅读
目的:探讨血清磷脂酰肌醇蛋白聚糖3(GPC3)对原发性肝细胞癌的诊断价值。方法:应用酶联免疫法测定35例肝细胞癌(HCC)、26例肝硬化和50例健康人血清GPC3和甲胎蛋白(AFP)水平,并分析比较两者对原发性HCC的诊断价值。结果:HCC组、肝硬化组和健康对照组血清GPC3分别为(4.16±2.35)、(3.13±1.19)和(1.92±1.23)ng·ml-1,HCC组明显高于肝硬化组和健康对照组,差异有统计学意义(分别P=0.018和P<0.001)。对于诊断HCC,GPC3的ROC曲线下面积为0.789,AFP为0.803;GPC3的敏感性为82.8%,特异性为56.5%;AFP的敏感性为62.8%,特异性为78.9%。GPC3与AFP联合应用诊断HCC则敏感性为91.4%,特异性为45.6%。对于Ⅰ期和Ⅱ期HCC,GPC3的阳性比例(11/14)高于AFP(4/14),差异有统计学意义(P=0.021)。结论:GPC3是一个敏感且特异的HCC标志物,检测血清GPC3有助于HCC的诊断与研究。
Objective: To investigate the value of serum glypican 3 (GPC3) in the diagnosis of primary hepatocellular carcinoma. Methods: The serum levels of GPC3 and AFP in 35 patients with hepatocellular carcinoma (HCC), 26 patients with cirrhosis and 50 healthy people were determined by enzyme-linked immunosorbent assay (ELISA), and their diagnostic value in primary HCC . Results: The serum GPC3 of HCC group, cirrhosis group and healthy control group were (4.16 ± 2.35), (3.13 ± 1.19) and (1.92 ± 1.23) ng · ml-1, respectively, which were significantly higher in HCC group than those in cirrhosis group and healthy controls Group, the difference was statistically significant (P = 0.018 and P <0.001, respectively). For the diagnosis of HCC, the area under the ROC curve of GPC3 was 0.789 and the AFP was 0.803; GPC3 had a sensitivity of 82.8% and a specificity of 56.5%; AFP had a sensitivity of 62.8% and a specificity of 78.9%. GPC3 and AFP combined diagnosis of HCC was 91.4% sensitivity and specificity of 45.6%. For stage I and stage II HCC, the positive rate of GPC3 (11/14) was higher than that of AFP (4/14), with a statistically significant difference (P = 0.021). Conclusion: GPC3 is a sensitive and specific HCC marker. Detecting serum GPC3 contributes to the diagnosis and research of HCC.