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观察牛磺酸对心肌细胞感染 ＣｏｘｓａｃｋｉｅＢ３病毒（ＣＶＢ３）后 Ｃａ２＋内流的影响。取新生 ＳＤ大鼠心室肌制备培养搏动心肌细胞， １８ ｈ后接种 １００ ＴＣＩＤ５０的 ＣＶＢ３作为实验性病毒性心肌炎模型，并采用放射性同位素４５Ｃａ２＋示踪技术，观察了 １～２０ ｍｍｏｌ／ Ｌ牛磺酸对正常及感染 ＣＶＢ３后心肌细胞 Ｃａ２＋内流的影响。结果：（ｌ）１０～２０ ｍｍｏｌ／ Ｌ牛磺酸对正常心肌细胞不同胞外 Ｃａ２＋浓度（［Ｃａ２＋］０， ０．５、 １．５和 ４．０ ｍｍｏｌ／ Ｌ）下的 Ｃａ２＋内流均有抑制作用，而 １ｍｍｏｌ／ Ｌ牛磺酸只抑制高［Ｃａ２＋］０（４．０ ｍｍｏｌ／ Ｌ）时 Ｃａ２＋内流，对低［Ｃａ２＋］０（０．５ ｍｍｏｌ／ Ｌ）和正常［Ｃａ２＋］０（１．５ ｍｍｏｌ／ Ｌ）时 Ｃａ２＋内流无明显作用；（２） ｌ～２０ ｍｍｏｌ／ Ｌβ－丙氨酸对 Ｃａ２＋内流无明显影响；（３）感染ＣＶＢ３后Ｃａ２＋内流增加，加用１ｍｍｏｌ／ Ｌ牛磺酸后能减少感染ＣＶＢ３后的Ｃａ２＋内流。结论：牛磺酸具有抑制培养心肌细胞感染ＣＶＢ３后Ｃａ２＋内流的作用，这可能是牛磺酸能减轻ＣＶＢ３所致细胞损伤的机理之一。
To observe the effect of taurine on Ca2 + influx in cardiomyocytes infected with Coxsackie B3 virus (CVB3). The cardiomyocytes of neonatal SD rats were used to prepare pulsed cardiomyocytes. CVB3 of 100 TCID50 was inoculated 18 hours later to induce the experimental myocarditis. The radioactive isotope 45Ca2 + tracer was used to observe the effects of 1 ~ 20 mmol / L taurine Effect of normal and CVB3 infection on Ca2 + influx in cardiomyocytes. Results: (1) Ca2 + influx from 10-20 mmol / L taurine to normal cardiomyocytes under different extracellular Ca2 + concentrations ([Ca2 +] 0, 0.5, 1.5 and 4.0 mmol / L) 1mmol / L taurine could inhibit the influx of Ca2 + in high [Ca2 +] 0 (4.0 mmol / L) (2) l ~ 20 mmol / L β-alanine had no significant effect on influx of Ca2 +; (3) Ca2 + influx increased after CVB3 infection and With 1mmol / L taurine can reduce the infection of CVB3 Ca2 influx. CONCLUSION: Taurine inhibits the influx of Ca2 + after CVB3 infection in cultured cardiomyocytes, which may be one of the mechanisms by which taurine can reduce cell injury induced by CVB3.