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bHLH是真核生物中重要的一类转录因子,其主要由碱性氨基酸区和螺旋-环-螺旋区组成。本研究利用生物信息学的方法鉴定到122个绿豆bHLH转录因子,并对其理化性质、保守结构域、基因结构、在染色体上的分布、系统进化以及部分典型基因的组织表达差异等进行分析。结果表明,bHLH转录因子理化性质差异较大;含有2个保守结构域,分别位于N端的碱性氨基酸区和C端的螺旋-环-螺旋区,碱性氨基酸区含有His5-Glu9-Arg13保守序列,与靶基因结合有关,HLH区含有Arg23和Arg55,与形成二聚体有关,同时含有5种保守元件;bHLH基因在11条染色体上分布不均匀,5号、7号和8号染色体上分布较多,1号、4号和10号染色体上分布较少,大部分基因含有1~9个不等的内含子,在染色体上成簇状分布;122个bHLH转录因子可分为11个亚家族。多数bHLH基因在绿豆根、茎、叶、花和种子等组织中均有表达,但具有组织表达特异性,且不同基因表达量差异较大。本研究为进一步研究绿豆bHLH转录因子家族的生物学功能奠定基础。
bHLH is an important class of transcription factors in eukaryotes, which mainly consists of basic amino acid regions and helix-loop-helix regions. In this study, 122 mung bean bHLH transcription factors were identified using bioinformatics methods, and their physical and chemical properties, conserved domains, gene structure, distribution on chromosomes, phylogenetic evolution and differences in tissue expression of some typical genes were analyzed. The results showed that the physical and chemical properties of bHLH transcription factor were quite different. The conserved domains of bHLH contained two conserved domains, located at the N-terminal basic amino acid region and C-terminal helix-loop-helix region, respectively. The basic amino acid region contained the conserved sequence of His5- Which is related to the binding of the target genes. The HLH region contains Arg23 and Arg55, which are related to the formation of dimers and contain five conserved components. The bHLH gene is unevenly distributed on chromosome 11 and the chromosomes on chromosomes 5, 7 and 8 There are few chromosomes on chromosomes 1, 4 and 10, most of them contain 1 to 9 introns, ranging from chromosome to chromosome; 122 bHLH transcription factors can be divided into 11 sub-populations family. Most bHLH genes are expressed in the roots, stems, leaves, flowers and seeds of mung bean, but they are tissue-specific and vary significantly in different gene expression. This study laid the foundation for further study on the biological function of mung bean bHLH transcription factor family.