１９９３年６～８月间，在河北省涿鹿县捕获部分蜱类，经分离与检测，从全沟硬蜱中肠分离出１株莱姆病螺旋体，以ＰＣＲ法从１只全沟硬蜱成蜱及１只日本血蜱若蜱体内检出莱姆病螺旋体ＤＮＡ阳性，从日本血蜱检出莱姆病螺旋体在国内外尚属首次。此外，我们还以ＩＦＡ、ＥＬＩＳＡ和Ｗｅｓｔｅｒｎｂｌｏｔ３种方法对５名可疑莱姆病患者血清进行了检测，１份血清ＩＦＡ试验ＩｇＧ抗体阳性，５份血清在Ｗｅｓｔｅｒｎｂｏｌｔ试验中出现的蛋白带有１～８条不等。 From June to August 1993, some ticks were captured in Zhuolu County of Hebei Province. After isolation and detection, one tick was isolated from the midgut of Ixodes latipes, It is the first time to detect Lyme disease spirochete from ticks and one Japanese tick. In addition, IFA, ELISA and Westernblot 3 methods were also used to detect the serum of 5 suspicious Lyme disease patients, 1 serum IFA test IgG antibody positive, 5 serum Westernbolt test protein appeared with 1 to 8 Do not wait.