目的 研究人巨细胞病毒(HCMV)感染对单核细胞及内皮细胞株表达趋化因子的影响,以及对两者之间趋化和黏附的影响。方法 通过ELISA法和半定量RT-PCR的方法,检测CMV感染对单核细胞株(THP-1)及内皮细胞株(ECV-304)几种趋化因子及其受体表达的改变,同时通过趋化实验和黏附实验研究内皮细胞对单核细胞的趋化和黏附的改变。结果 HCMV感染可刺激ECV-304分泌MCP-1、GROα,在mRNA水平可改变内皮细胞MCP-1、CRO-α和IL-8表达,先下调后上调(IL-8除外);而对单核细胞趋化因子受体CCR2、CXCR2的表达在mRNA水平却有明显的下调作用(P<0.05)。HCMV感染可显著增强内皮细胞对单核细胞的趋化和黏附。结论 HCMV感染可增强内皮细胞对单核细胞的趋化和黏附,参与炎症反应,这可能与CMV上调内皮细胞趋化因子表达有关。 Objective To study the effect of human cytomegalovirus (HCMV) infection on the expression of chemokines in monocytes and endothelial cell lines, as well as on the chemotaxis and adhesion between them. Methods The expression of several chemokines and their receptors in CMV-infected monocytic cell line (THP-1) and endothelial cell line (ECV-304) were detected by ELISA and semi-quantitative RT- Chemotaxis and adhesion experiments were performed to study the chemotaxis and adhesion of endothelial cells to monocytes. Results HCMV infection stimulated the secretion of MCP-1 and GROα by ECV-304, and up-regulated the expression of MCP-1, CRO-α and IL-8 in endothelial cells at the mRNA level except for IL- The expressions of CCR2 and CXCR2 were significantly downregulated at mRNA level (P <0.05). HCMV infection can significantly enhance the chemotaxis and adhesion of endothelial cells to monocytes. Conclusion HCMV infection can enhance the chemotaxis and adhesion of endothelial cells to monocytes and participate in inflammatory reaction, which may be related to the up-regulation of chemokine expression of endothelial cells by CMV.