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目的 :探讨缺氧对体外培养鼠脑微血管内皮细胞和星形胶质细胞分泌组织型纤溶酶原激活物 ( tissue- typeplasminogen activator,TPA)的影响。方法 :分别对新生小鼠脑微血管内皮细胞、星状胶质细胞进行缺氧条件下培养 ,常规培养作为空白对照 ,每组各取 8例 ,吸取培养液用酶联免疫吸附试验测试 TPA活性。结果 :缺氧后内皮细胞 TPA活性明显增高 ( P<0 .0 1) ,星形胶质细胞 TPA活性无明显变化 ( P>0 .0 5)。结论 :体外培养鼠脑微血管内皮细胞和星形胶质细胞均能合成分泌 TPA;缺氧状态下脑微血管内皮细胞产生的 TPA活性增高。内皮细胞分泌的TPA是脑缺氧缺血致不可逆神经元损伤的一个重要媒介 ,而星形胶质细胞分泌的 TPA则主要参与发育阶段需要细胞迁移的重建活动
Objective: To investigate the effect of hypoxia on the secretion of tissue-type plasminogen activator (TPA) in cultured rat cerebral microvascular endothelial cells and astrocytes. Methods: Cerebral microvascular endothelial cells and astrocytes of neonatal mice were cultured under hypoxia condition. Conventional culture was used as a blank control. Eight rats in each group were taken. The activity of TPA was tested by enzyme-linked immunosorbent assay (ELISA). Results: After hypoxia, the activity of TPA in endothelial cells was significantly increased (P <0.01), and the activity of TPA in astrocytes did not change significantly (P> 0.05). CONCLUSION: TPA can be synthesized and secreted in cultured rat brain microvascular endothelial cells and astrocytes in vitro. TPA activity in endothelial cells under hypoxia is increased. TPA secreted by endothelial cells is an important mediator of irreversible neuronal damage induced by hypoxic-ischemic brain injury. Astrocytes secrete TPA, which is mainly involved in the reconstruction of cells that require cell migration at the developmental stage