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目的 探讨一氧化氮 (NO)和血管紧张素Ⅱ (AngⅡ )在早期糖尿病大鼠肾小球高滤过状态中的作用。 方法 利用链脲佐菌素诱导大鼠实验性糖尿病模型 ,将大鼠分为正常对照组、未治疗组、卡托普利组及左旋硝基精氨酸甲基酯 (L NAME)组 ,观察 4周后糖尿病大鼠肾脏血流动力学和血浆、肾组织匀浆中NO、AngⅡ浓度的改变。结果 糖尿病未治疗组较正常对照组的肾重 /体重、2 4h尿蛋白、内生肌酐清除率、肾小球滤过率、肾血浆流量、血清及肾组织匀浆中NO-3 和AngⅡ活性显著升高 (P <0 .0 5或P <0 .0 1)。卡托普利组 ,除血浆及肾组织匀浆中NO-3 未检测外 ,上述各项水平均有不同程度下降 (P <0 .0 5或P <0 .0 1)。L NAME组 ,除血浆及肾组织匀浆中AngⅡ未检测及 2 4h尿蛋白无明显下降外 ,余项均有显著下降 (P <0 .0 5或P <0 .0 1)。结论 糖尿病早期血浆NO、AngⅡ升高可造成肾小球高功能状态 ,用卡托普利及L NAME可减轻糖尿病早期肾脏的高功能状态 ,延缓糖尿病肾病的发生与发展
Objective To investigate the role of nitric oxide (NO) and angiotensin Ⅱ (Ang Ⅱ) in glomerular hyperfiltration in early diabetic rats. Methods Streptozotocin-induced diabetic model rats were divided into normal control group, untreated group, captopril group and L-NAME group, observed After 4 weeks, the hemodynamics of kidney and NO, Ang Ⅱ concentration in plasma and kidney homogenate of diabetic rats were observed. Results Compared with the normal control group, the urinary protein, creatinine clearance rate, glomerular filtration rate, renal plasma flow, NO-3 and AngⅡ activities in serum and kidney homogenate of diabetic untreated group were significantly higher than those of normal control group Was significantly higher (P <0.05 or P <0.01). Captopril group, in addition to the plasma and renal homogenate NO-3 was not detected, the above levels were decreased to varying degrees (P <0.05 or P <0.01). L NAME group, the remaining items were significantly decreased (P <0.05 or P <0.01) except that AngⅡ was not detected in plasma and renal homogenate and no significant decrease in 24 h urinary protein. Conclusion In the early stage of diabetes mellitus, NO and AngⅡincrease the high glomerular state. Using captopril and L NAME can reduce the high-function state of early diabetic nephropathy and delay the occurrence and development of diabetic nephropathy