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目的 探讨卵巢癌患者的细胞免疫状态以及 IL- 2 ,血清游离白细胞介素 - 2受体 (s IL- 2 R) ,外周血单个核细胞(PBMC)膜结合型白细胞介素 - 2受体 (m IL- 2 R)与临床的关系 .方法 3H- Td R参入法测 31例卵巢癌患者 IL- 2产生水平 ,EL ISA法检测血清 s IL - 2 R水平 ,APAAP免疫组化检测外周血单个核细胞膜结合型 IL - 2 R表达细胞 (CD2 5 + 细胞 ) .结果 31例卵巢恶性肿瘤患者术前及术后 7d,IL- 2产生能力降低 ,s IL- 2 R水平增高 ,与术后第 6疗程化疗结束后 4wk相比有显著差异 (P<0 .0 1) ;术前临床 ～ 期患者 IL- 2活性低于 ～ 期患者 ,s IL- 2 R水平明显高于 ～ 期患者 (P<0 .0 5 ) ;CD2 5 + 细胞均为个别阳性 ,各组间无差异 ;术后复发的 5例患者血清 s IL - 2 R持续增高 .结论 对 IL - 2及 s IL - 2 R的定期检测 ,有助于观察卵巢恶性肿瘤患者病情变化 ,为其预后判断提供重要参考
Objective To investigate the cellular immune status of patients with ovarian cancer and the effects of interleukin - 2 (IL - 2), serum interleukin - 2 receptor (sIL - 2R), peripheral blood mononuclear cells (PBMC) m IL-2 R) in patients with ovarian cancer.Methods IL-2 production was measured in 31 cases of ovarian cancer patients by 3H-TdR incorporation method.Serum levels of sIL-2R were detected by ELISA, APAAP immunohistochemical detection of peripheral blood mononuclear cells (CD25 + cells) .Results The production of IL-2 and the level of sIL-2R in 31 patients with ovarian cancer before operation and 7 days after operation were significantly higher than those after operation The level of IL-2R was significantly higher in patients with stage ~ 6 than those in stage ~ 6 (P <0. 01) <0.05). CD2 5 + cells were all positive and there was no difference between the two groups.The serum level of sIL - 2R in 5 patients relapsed after operation continued to increase.Conclusion The IL - 2 and sIL - 2R Regular testing can help observe the changes of patients with ovarian cancer, provide an important reference for the prognosis