论文部分内容阅读
目的:研究三种低氧训练模式对大鼠血液运氧能力的影响。方法:70只雄性SD大鼠,经过适应性训练和力竭实验筛选出40只,平均分成4组,保证每组大鼠体重、力竭时间、力竭后血乳酸基本一致,采用双盲法分为:对照组、高住高练组、高住低练组和低住高练组。采用水平动物跑台进行耐力训练,常氧下运动强度为35m/min,低氧下运动强度为30m/min,1h/天,5天/周,持续训练6周。最后一次训练后恢复48h腹主动脉取血。采用全血分析仪测定血液RBC、Hb和Hct,分光光度计测定血液2,3-二磷酸甘油酸(2,3-DPG)含量。结果:与对照组比较,高住高练组大鼠血液RBC、Hb、Hct和2,3-DPG显著升高;高住低练组和低住高练组大鼠RBC、Hb、Hct和2,3-DPG与对照组比较无显著性差异。结果提示,就提高机体运氧能力而言,高住高练效果优于高住低练和低住高练。
Objective: To study the effects of three hypoxic training modes on blood oxygenation in rats. Methods: Seventy male Sprague-Dawley rats were screened out with adaptive training and exhaustive exercise. Forty rats were randomly divided into four groups. Body weight, exhaustion time and blood lactic acid after exhaustive exercise in each group were basically the same. Double-blind Divided into: the control group, live high living training group, living high and low training group and live in training group. The endurance training was carried out by horizontal animal treadmill. The exercise intensity under normoxia was 35m / min, the exercise intensity under hypoxia was 30m / min, 1h / day, 5 days / week, continuous training for 6 weeks. Abdominal aorta blood was recovered 48h after the last training. Blood RBC, Hb and Hct were measured using a whole blood analyzer. The content of 2,3-diphosphoglycerate (2,3-DPG) in the blood was determined by spectrophotometer. Results: Compared with the control group, the blood RBC, Hb, Hct and 2,3-DPG levels were significantly increased in high-living-high training group rats; RBC, Hb, Hct and 2 , 3-DPG compared with the control group no significant difference. The results suggest that, in terms of improving the body’s ability to transport oxygen, live-in and go-ahead are better than live-in and live-in.