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天然氨基酸(NAA)是一种具有良好生物相容性的材料,对其进行功能化设计是近年来的热门研究领域。本文中将基于蚕丝蛋白的特征氨基酸序列(Gly-Ala)与离子互补多肽序列(Arg-Ala-Asp-Ala)混编,设计了多肽RAG-16。采用原子力显微镜、旋转流变仪、傅立叶变换红外光谱仪、倒置荧光显微镜等技术对多肽RAG-16的自组装结构、流变学性能以及细胞相容性等性质进行了表征。结果表明:多肽RAG-16在溶液中具有自组装特性,能够形成纳米级三维网络结构,所形成的水凝胶力学性能较佳。通过分析得知,多肽二级结构中Silk I结构比例增加是其力学性能增强的主要原因。荧光染色显示,绝大多数接种于多肽水凝胶中的MC3T3-E1细胞能够存活,且能在不同的三维平面上生长增殖,表明该材料具有良好的细胞相容性。综上所述,本实验中设计的NAA材料在生物医学领域有较大的应用潜力。
Natural amino acid (NAA) is a kind of material with good biocompatibility. Its functional design has been a hot research field in recent years. In this paper, the polypeptide RAG-16 was designed based on the hybridization of the amino acid sequence (Gly-Ala) of silk protein with the sequence of Arg-Ala-Asp-Ala. The self-assembly structure, rheological properties and cell compatibility of peptide RAG-16 were characterized by atomic force microscopy, rotational rheometer, Fourier transform infrared spectroscopy and inverted fluorescence microscope. The results showed that the polypeptide RAG-16 has the self-assembly property in solution and can form a nano-scale three-dimensional network structure, and the formed hydrogel has better mechanical properties. Through the analysis, it is found that the increase of the proportion of Silk I structure in the secondary structure of the polypeptide is the main reason for its enhanced mechanical properties. Fluorescent staining showed that the vast majority of MC3T3-E1 cells seeded in the peptide hydrogel survived and proliferated in different three-dimensional planes, indicating that the material has good cytocompatibility. In summary, the NAA material designed in this experiment has great potential in the field of biomedicine.