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利用琼脂糖凝胶电泳法研究了钌(Ⅱ)多吡啶配位物[Ru(bpy)2(ODCIP)]2+(bpy:2,2’-联吡啶,ODCIP:含多个配位中心的多吡啶配体,3,4-二氯基-咪唑并[4,5-f][1,10]邻菲咯啉)对pBR322质粒DNA的光切割作用及其可能机理,并运用紫外可见吸收光谱、荧光光谱和琼脂糖凝胶电泳法研究了[Ru(bpy)2(ODCIP)]2+与Zn2+配位后与DNA的光谱性质和光切割作用.结果表明[Ru(bpy)2(ODCIP)]2+对DNA有较好的光切割作用,其机理可能是产生了超氧阴离子自由基和单线态氧.[Ru(bpy)2(ODCIP)]2+与Zn2+配位可能形成的双核配位物[Ru(bpy)2(ODCIP)Zn]4+与DNA也能进行插入结合,对DNA的光切割效果并没有明显增强。
The ruthenium (Ⅱ) polypyridine ligand [Ru (bpy) 2 (ODCIP)] 2+ (bpy: 2,2’-bipyridine, ODCIP Photoprocessing of pBR322 plasmid DNA with polypyridyl ligand, 3,4-dichloro-imidazo [4,5-f] [1,10] phenanthroline and its possible mechanism were studied. UV-Vis absorption The spectral properties and photodissociation of DNA with [Ru (bpy) 2 (ODCIP)] 2+ and Zn2 + were studied by spectroscopic, fluorescent and agarose gel electrophoresis. The results showed that [Ru (bpy) 2 ] 2+ has a better photodissociation effect on DNA, which may be due to the production of superoxide anion radical and singlet oxygen. [Ru (bpy) 2 (ODCIP)] 2+ The positioner [Ru (bpy) 2 (ODCIP) Zn] 4+ can also be inserted into the DNA and the light-cutting effect on DNA is not significantly enhanced.