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目的:探求早期、快速和准确诊断真菌性角膜炎的实验室方法。方法:对58例真菌培养阳性的真菌性角膜炎患者的角膜刮片进行涂片镜检,同一涂片先行KOH湿片镜检,然后行Calcofluor White(CFW)荧光染色镜检。以真菌培养阳性为诊断真菌性角膜炎的金标准,对检查结果进行对比分析。结果:同一涂片KOH湿片阳性率为81.0%(47/58),而CFW荧光染色阳性率为96.6%(56/58),2者共阳性47例,共阴性2例,CFW荧光染色阳性率高于KOH湿片镜检法(χ2=7.11,P<0.001)。结论:角膜刮片CFW荧光染色镜检诊断真菌性角膜炎优于KOH湿片镜检。
Objective: To explore an early, rapid and accurate laboratory method for the diagnosis of fungal keratitis. Methods: Fifty-eight cases of fungal keratitis with fungal culture were examined by smear microscopy. The same smears were examined by KOH wet-scanning microscope and Calcofluor White (CFW) fluorescence microscopy. Fungal culture positive for the diagnosis of fungal keratitis gold standard, comparative analysis of test results. Results: The positive rate of KOH wet patch in the same smear was 81.0% (47/58), while the positive rate of CFW fluorescence staining was 96.6% (56/58). There were 47 common positives and 2 negatives in total. The positive staining of CFW was positive Rate was higher than that of KOH wet film microscopy (χ2 = 7.11, P <0.001). Conclusions: Fungal keratitis is better than corneal KOH in corneal scraping by CFW fluorescence microscopy.