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目的研究反义核酸的抗病毒作用。方法设计合成了针对鸭乙型肝炎病毒（ＤＨＢＶ）前Ｓ（ＰｒｅＳ）基因区第９５１９６８位核苷酸的硫代反义寡脱氧核苷酸（ＡＳＯＤＮ），以２０μｇ／ｇ体重／日剂量对３只腹腔感染ＤＨＢＶ５２毒株后，血清ＤＨＢｓＡｇ及ＤＨＢＶＤＮＡ阳性鸭连续静脉注射１０天，同时以等体积生理盐水注射另３只感染鸭作为对照。结果对照鸭注射生理盐水后，血清ＤＨＢｓＡｇ及ＤＨＢＶＤＮＡ阳性未见明显改变，肝组织ＤＮＡＳｏｕｔｈｅｒｎ杂交在３０与２３ｋｂ左右杂交信号明显。注射ＡＳＯＤＮ鸭在１０天后，２／３鸭血清ＤＨＢｓＡｇ及ＤＨＢＶＤＮＡ量显著降低，３／３鸭肝组织中３０ｋｂ左右的杂交信号显著减弱，２３ｋｂ左右未见杂交信号。结论说明该段ＡＳＯＤＮ在鸭体内能部分抑制ＤＨＢＶ的复制与抗原表达。
Objective To study the antiviral effect of antisense nucleic acid. Methods The antisense oligodeoxynucleotides (ASODN) of 951-968 nucleotides of preS gene region of duck hepatitis B virus (DHBV) were designed and synthesized. Daily dose of DHBV5.2 strain infected with three intraperitoneal, serum DHBsAg and DHBVDNA positive ducks for continuous intravenous injection of 10 days, while the same volume of normal saline injection of the other three infected duck as a control. Results Compared with the control group, the DHBsAg and DHBVDNA of the control group showed no significant change after injection of saline, and the hybridization signal of DNASouthern hybridization was found between 3 0 and 2 3kb. Serum DHBsAg and DHBVDNA were significantly decreased in 2/3 ducklings after injection of ASODN ducklings, and the hybridization signal of 3 0kb in 3/3 duck liver tissues was significantly weakened. No hybridization signal was found in about 23kb ducklings. The results show that the AS-ODN in this section of duck can partially inhibit DHBV replication and antigen expression.