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目的探讨血吸虫病肝硬化门静脉高压症时肺组织的病理变化和内皮素1(ET1)、NO在肺血管病变机制中的作用。方法运用腹部敷贴法感染血吸虫尾蚴制作大耳白兔肝硬化门静脉高压症动物模型(n=10),采用免疫组化技术和HE染色、Masson三色染色及透射电镜方法研究血吸虫病肝硬化门脉高压症兔肺组织病理改变和ET1、NO合酶(NOS)的表达、分布。结果门脉高压症兔肺组织中ET1、NOS阳性或强阳性表达伴病理学改变,正常兔为阴性或弱阳性,图像定量分析两组灰度值和吸光度值差异具有统计学意义(P<0.01)。结论血吸虫病肝硬化门脉高压症时兔肺组织发生明显的病理改变,ET1、NO可能在血吸虫病门脉高压症肺血管病变发病机制中具有重要意义。
Objective To investigate the pathological changes of lung tissue and the role of endothelin-1 (ET1) and NO in the pathogenesis of pulmonary vascular disease in patients with schistosomiasis cirrhosis and portal hypertension. Methods Abdominal plaster was used to infect rabbits with cirrhosis and portal hypertension caused by Schistosoma japonicum (n = 10). Immunohistochemistry, HE staining, Masson trichrome staining and transmission electron microscopy were used to study the effects of schistosomiasis cirrhosis Pathological changes of lung tissue and expression and distribution of ET1 and NO synthase (NOS) in rabbit with venous hypertension. Results The positive or negative expression of ET1 and NOS in the pathological changes of lung tissue of rabbits with portal hypertension were negative or weakly positive. The difference of the gray value and the absorbance between the two groups was statistically significant (P <0.01 ). Conclusion Schistosoma japonicum cirrhosis and portal hypertension have obvious pathological changes in lung tissue. ET1 and NO may play an important role in the pathogenesis of pulmonary vascular disease in schistosomiasis patients with portal hypertension.