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目的:探讨核心蛋白聚糖(DCN)对人胃癌细胞SGC-7901裸鼠移植瘤生长的影响及可能的机制。方法:构建裸鼠胃癌移植瘤模型,将重组质粒pEGFP-N1-DCN注入肿瘤中央及基底部,以空质粒组和生理盐水组作为对照,测量肿瘤体积和质量的变化,RT-PCR及Western blot法检测DCN的表达,Western blot法检测转化生长因子-β1(TGF-β1)和基质金属蛋白酶-9(MMP-9)的表达,免疫组织化学染色方法检测血管内皮生长因子(VEGF)的表达。结果:裸鼠胃癌移植瘤模型构建成功,与对照组比较,重组质粒组肿瘤生长速度减慢,肿瘤体积和质量明显变小(P<0.05);RT-PCR显示重组质粒组DCN表达增强(P<0.05),Western blot结果显示重组质粒组有融合蛋白的表达,重组质粒组TGF-β1及MMP-9蛋白表达均降低(P<0.05);免疫组化显示重组质粒组VEGF表达降低(P<0.05)。结论:DCN基因可以抑制裸鼠移植瘤生长,其机制可能与转化生长因子-β1及基质金属蛋白酶-9蛋白的表达降低和肿瘤新生血管形成抑制有关。
Objective: To investigate the effect of decorin (DCN) on the growth of human gastric cancer SGC-7901 xenografts in nude mice and its possible mechanism. Methods: The model of gastric cancer xenografts in nude mice was established. The recombinant plasmid pEGFP-N1-DCN was injected into the central and basal part of the tumor. The empty plasmid group and the normal saline group were used as control to measure the tumor volume and quality. RT-PCR and Western blot The expression of DCN was detected by Western blot. The expression of transforming growth factor-β1 (TGF-β1) and matrix metalloproteinase-9 (MMP-9) was detected by Western blotting. The expression of VEGF was detected by immunohistochemical staining. Results: The model of gastric cancer xenografts in nude mice was established successfully. Compared with the control group, the tumor growth rate of the recombinant plasmid group was slowed down and the tumor volume and quality were significantly decreased (P <0.05). The expression of DCN in the recombinant plasmid group was enhanced by RT-PCR (P <0.05). The result of Western blot showed that there was fusion protein expression in the recombinant plasmid group. The expression of TGF-β1 and MMP-9 protein in the recombinant plasmid group were decreased (P <0.05) 0.05). Conclusion: The DCN gene can inhibit the growth of xenografts in nude mice. The mechanism may be related to the decreased expression of TGF-β1 and MMP-9 and the inhibition of tumor angiogenesis.