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对实验性缺血的大鼠心肌切片进行免疫组化染色，检测肌酸激酶。结果显示，在心肌缺血４０ｍｉｎ后，着色开始呈现酶活性减少的变化，并随缺血时间延长而更加明显。实验证实肌酸激酶的免疫组化染色，是一种诊断早期心肌缺血的特异性强、敏感性高的重要辅助方法。
The ischemic rat myocardial sections were immunohistochemically stained to detect creatine kinase. The results showed that after 40min of myocardial ischemia, the change of enzymatic activity decreased with the onset of ischemia, and became more obvious with the prolongation of ischemia. Experiments confirmed that creatine kinase immunohistochemical staining is a diagnosis of early myocardial ischemia strong specificity and high sensitivity of the auxiliary method.