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为了揭示河北省苹果树腐烂病病菌的群体遗传多样性,应用5条随机引物对采自河北省张家口、石家庄、邢台、廊坊、衡水、秦皇岛、唐山、沧州和保定9个苹果产区的、具有代表性的44个苹果树腐烂病病菌分离株的DNA进行了RAPD分析。结果表明:这些分离株共产生了89条谱带,并且均为多态性条带,多态性位点率为100%;应用NTSYS 2.1软件对所得到的RAPD图谱进行聚类分析,以遗传距离0.72为阈值,所有的菌株可划分为7个遗传群体,这说明河北省苹果树腐烂病病菌具有丰富的种内遗传多样性,然而,这种群体多样性与地理来源并无直接关系;鉴于黄色、浅黄色和灰白色菌株为河北省苹果树腐烂病病菌的优势菌株,特选出分别来自第Ⅰ~Ⅶ组的6号、8号、27号、37号、14号、20号和31号菌株作为备用菌株,供今后在抗病种质材料测试中使用。
In order to reveal the population genetic diversity of apple tree rot pathogen in Hebei province, five random primers were used to detect the genetic diversity of apple tree rot pathogen in Hebei province. The five random primers were collected from nine apple producing areas in Zhangjiakou, Shijiazhuang, Xingtai, Langfang, Hengshui, Qinhuangdao, Tangshan, Cangzhou and Baoding in Hebei Province, Representative DNA from 44 apple tree rot disease isolates were analyzed by RAPD. The results showed that 89 bands were produced by these isolates and all of them were polymorphic bands with a polymorphic locus rate of 100%. Cluster analysis of the RAPD patterns was performed using NTSYS 2.1 software, The distance of 0.72 was the threshold value. All the isolates could be divided into seven genetic groups, which indicated that the pathogen of apple tree rot disease in Hebei Province was rich in intraspecific genetic diversity. However, this population diversity was not directly related to geographical origin; Yellow, light yellow and gray strains are the dominant strains of apple tree rot pathogen in Hebei Province, and 6, 8, 27, 37, 14, 20 and 31 Strain as a backup strain for future use in disease resistance germplasm testing.