本文介绍了香连丸的紫外和荧光扫描测定法。样品中生物碱经甲醇-盐酸提取后,用正丁醇-醋酸-水在硅胶薄层板上分离,得到小檗碱斑点,直接测定其紫外吸收或荧光强度,其结果满意。两种测试方法简便快速,准确可靠。荧光法较紫外法灵敏得多,可用于微量成分测定。 This article describes the ultraviolet and fluorescence scanning assays of Xianglian Pills. After the alkaloids were extracted with methanol-hydrochloric acid, they were separated on a thin layer of silica gel with n-butanol-acetic acid-water to obtain berberine spots. The ultraviolet absorption or fluorescence intensity was directly measured. The results were satisfactory. The two test methods are simple, fast, accurate and reliable. The fluorescence method is much more sensitive than the ultraviolet method and can be used for the determination of trace components.