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本实验旨在了解化疗药物和细胞因子协同作用对细胞凋亡的影响。在培养的小鼠T淋巴瘤RMA细胞系中加化疗药物地塞米松 (DEX)、足叶乙甙 (VP16 )、三氧化二砷 (As2 O3 )及维甲酸(ATRA)以及培养细胞中先分别与细胞因子IL 2 ,IL 6和GM CSF共同培养后再加入上述药物 ,观察对引起细胞凋亡的影响。四种化疗药均能抑制RMA细胞增殖 ,DEX ,VP16和As2 O3 可以诱导细胞凋亡 ,单独使用ATRA不能引起RMA细胞凋亡 ,但与DEX联用时可降低DEX诱导凋亡的浓度。单用IL 2 ,IL 6或GM CSF不引起肯定的细胞凋亡 ;如同时并用IL 2和IL 6则出现肯定的细胞凋亡。化疗药物与细胞因子并用时可减低药物引发细胞凋亡的浓度 ,并使细胞凋亡发生的时间提前。实验结果表明 ,化疗药物与细胞因子在诱导细胞凋亡中具有协同作用 ,为细胞因子特别是IL 2与化疗药物配伍使用治疗恶性淋巴瘤提供了一定实验依据。
The purpose of this experiment was to understand the effects of synergistic effects of chemotherapeutic drugs and cytokines on apoptosis. The chemotherapeutic drugs dexamethasone (DEX), etoposide (VP16), arsenic trioxide (As2O3), and retinoic acid (ATRA) and cultured cells were first added to cytokines in cultured mouse T-lymphoma RMA cell lines. After IL 2, IL 6, and GM CSF were co-cultured, the above drugs were added and the effect of causing apoptosis was observed. All four chemotherapy drugs can inhibit RMA cell proliferation, DEX, VP16 and As2O3 can induce apoptosis, ATRA alone can not cause apoptosis of RMA cells, but combined with DEX can reduce the concentration of DEX induced apoptosis. IL 2 , IL 6 or GM CSF alone did not cause apoptotic cell death; if both IL 2 and IL 6 were used together, positive apoptosis occurred. When chemotherapeutic drugs and cytokines are used together, the concentration of apoptosis induced by the drug can be reduced, and the time of occurrence of apoptosis can be advanced. The experimental results show that chemotherapeutic drugs and cytokines have a synergistic effect in inducing apoptosis, and provide a certain experimental basis for the use of cytokines, especially IL 2 and chemotherapeutic drugs for the treatment of malignant lymphoma.