苹果锈果病是我国北部苹果栽培区的重要病害之一。業已証明,这是一种可以用嫁接传染的病毒病害。由于症状只表現在果实上以及少数品种的幼苗叶片上,因此对于未結果的果树和無叶片症状的苗木的鉴别十分困难。寻求一种鑒別病苗或健苗的方法对检疫来說十分必要。过去會經試驗一种化学診断法但是效果不稳定。有些診断法都是依据蛋白質,多酚化合物或还元糖的量或質的改变而设计的。但当改变的量極为微小时,一般的反应剂很难显示出健株和病株的区别。如所周知,病毒蛋白質往往显示出氨基酸含量的 Apple rust fruit disease is one of the important diseases in the apple cultivation area in northern China. It has been shown that this is a viral disease that can be spread by grafting. Because symptoms are present only on fruits and on a few varieties of seedling leaves, identification of unproductive fruit trees and leaf-free seedlings is very difficult. The quest for a method of identifying sick or healthy seedlings is essential for quarantine. In the past, a chemical diagnosis was tested but the effect was not stable. Some diagnostics are based on changes in the amount or quality of a protein, polyphenolic compound, or sugar. However, when the amount of change is extremely small, it is very difficult for the typical reagents to show the difference between healthy and diseased plants. As we all know, viral proteins often show amino acid content