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目的 :探讨三氧化二砷 (As2 O3)对H2 2肝癌荷瘤小鼠免疫功能的影响及实体瘤生长情况。方法 :利用流式细胞仪及改进的MTT还原法分别对正常小鼠、荷瘤小鼠对照组、荷瘤小鼠As2 O3治疗组进行T细胞亚群及NK细胞活性 (NKCA)的测定 ,并比较各组瘤重 ,计算瘤重抑制率。结果 :肝癌小鼠荷瘤对照组与正常组比较 ,CD3 +,CD4+,CD4+ CD8+及NKCA均明显减少 (P <0 .0 1) ;低剂量及高剂量As2 O3治疗组与荷瘤对照组比较 ,CD3 +,CD4+,CD4+ CD8+及NKCA均明显升高 (P <0 .0 5 ) ,CD8+无明显变化 ;而高剂量As2 O3治疗组与低剂量As2 O3治疗组比较 ,CD4+,NKCA明显升高 (P <0 .0 5 ) ,CD3 +,CD4+ CD8+无明显变化。治疗组瘤重抑制率分别为 3 9.12 % ,45 .74% ,病理观察可见间质内大量炎症细胞浸润。结论 :AS2 O3能抑制实体肿瘤的生长 ,提高肝癌小鼠的免疫功能 ,这是一值得继续研究的临床课题
Objective: To investigate the effect of arsenic trioxide (As2 O3) on the immune function of H22 tumor-bearing mice and the growth of solid tumors. Methods: T cell subsets and NK cell activity (NKCA) were measured by flow cytometry and modified MTT reduction method in normal mice, tumor-bearing mice control group and tumor-bearing mice As2 O3 treatment group respectively The tumor weight was compared to calculate the tumor weight inhibition rate. Results: Compared with the normal control group, the number of CD3 +, CD4 +, CD4 + CD8 + and NKCA in hepatocellular carcinoma mice was significantly decreased (P <0.01). Compared with the tumor-bearing control group, the low and high dose As2 O3 treatment groups CD3 +, CD4 +, CD4 + CD8 + and NKCA were significantly increased (P <0.05), while there was no significant change in CD8 + cells. However, CD4 + and NKCA were significantly increased in high dose As2O3 treated group compared with low dose As2O3 treated group (P <0. 05), CD3 +, CD4 + CD8 + no significant change. The treatment group tumor weight inhibition rates were 3 9.12%, 45.74%, the pathological observation showed a large number of interstitial inflammatory cell infiltration. CONCLUSIONS: AS2 O3 inhibits the growth of solid tumors and enhances the immune function in mice with hepatocellular carcinoma, a clinical issue worth further study