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目的：为进一步分析ＴＣＶ免疫诱导同种免疫反应低下的机制。方法：采用Ｂ细胞杂交瘤技术获得分泌单克隆抗体的杂交瘤细胞。结果：两次的细胞融合中共得到１２株稳定分泌单抗的杂交瘤细胞，为分析抗体在ＴＣＶ中的作用提供条件。结论：ＴＣＶ免疫可引起抗ＴＣＶ细胞抗体的产生，以同系免疫的方法得到的活化Ｂ细胞用于Ｂ细胞杂交生产单抗是可行的。
OBJECTIVE: To further analyze the mechanism of immune-induced immune rejection induced by TCV. Methods: Hybridoma cells secreting monoclonal antibodies were obtained by B cell hybridoma technique. RESULTS: Twelve hybridomas secreting McAbs were obtained in the two cell fusions, which provided conditions for analyzing the role of antibodies in TCV. CONCLUSION: TCV immunization can induce the production of anti-TCV antibody. It is feasible to use activated B cells obtained by the method of homologous immunization for B cell hybridization to produce monoclonal antibodies.