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实时荧光定量ＰＣＲ方法 (ＦＱ ＰＣＲ)克服了已有的定量ＰＣＲ方法的缺点 ,特异性强 ,定量准确[1] 。我们应用实时荧光定量ＲＴ ＰＣＲ(ＦＱ ＲＴ ＰＣＲ)方法对 β地中海贫血 (β地贫 )患者 β与γ珠蛋白ｍＲＮＡ水平进行检测 ,现将结果报告如下。对象和方法1 对象1.1 β地
Real-time fluorescence quantitative PCR method (FQ PCR) overcomes the shortcomings of the existing quantitative PCR methods, with strong specificity and accurate quantification [1]. We used real-time fluorescence quantitative RT-PCR (FQ RT PCR) methods to detect β and γ-globin mRNA levels in patients with β-thalassemia (β thalassemia) and the results are reported below. Objects and methods 1 object 1.1 β earth