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采用不同压力条件体外培养人脐静脉血管内皮细胞(HUVECs),观察中药葛根素(Puerarin) 对内皮细胞产生—氧化氮(NO)和血管紧张素转换酶(ACE)活性的影响.结果:与大气压对照组相比,中压力对照组的NO和ACE均增加(P<0.05),而高压力对照组中NO含量显著减少,ACE活性则明显增加(P<0.O1);加葛根素干预后,中压力组中葛根素Ⅱ与对照组相比,NO的量明显增加,ACE活性明显下降,差异均有显著性意义(P<0.05);高压力组中葛根素Ⅰ和Ⅱ与对照组比较,NO明显增加,ACE则显著下降,差异均有显著性意义(P<0.05、P<0.01).结论:中压力促使VECs产生NO和ACE增加,高压力使VECs产生NO减少,ACE活性增加;葛根素能抑转高压对VECs产生NO和ACE活性的作用.
Human umbilical vein endothelial cells (HUVECs) were cultured in vitro under different pressure conditions to observe the effect of Chinese traditional medicine Puerarin on endothelial cell production of nitric oxide (NO) and angiotensin converting enzyme (ACE) activity. Result: Atmospheric pressure Compared with the control group, the NO and ACE in the middle pressure control group increased (P<0.05), while the NO content in the high pressure control group decreased significantly, and the ACE activity increased significantly (P<0.O1); after intervention with puerarin Compared with the control group, the amount of NO in the puerarin II group was significantly higher than that in the control group, and the ACE activity was significantly decreased (P<0.05); Puerarin I and II in the high-stress group were compared with the control group. , NO increased significantly, ACE decreased significantly, the difference was significant (P<0.05, P<0.01). Conclusion: The middle pressure promotes the increase of NO and ACE in VECs, and high pressure makes VECs reduce NO and increase ACE activity. Puerarin can inhibit the effects of high pressure on NO and ACE activity in VECs.