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为了研究广藿香内生真菌Diaporthe longicolla A616的活性次级代谢产物。采用正相硅胶、C18反相、Sephadex LH-20凝胶、HPLC等色谱技术从该菌株的发酵产物中分离得到了10个化合物,经波谱数据分析和文献比对,分别鉴定为1,3-diamino-1,3-dimethylurea(1),(7R,9R)-7-hydroxy-9-propyl-5-nonen-9-olide(2),ergosta-5,7,22-trien-3β-ol(3),(22E,24R)-ergosta-4,6,8(14)-22-tetraen-3-one(4),(22E,24R)-3β,5α-dihydroxy-6β-ergosta-7,22-diene(5),citreoisocoumarin(6),glycerol monolinoleate(7),1-(2-hydroxyethoxy)ethyl(E)-octadec-9-enoate(8),cyclo-(L-Pro-L-Ala)(9),cyclo(L)-Pro-(L)-Val(10),其中,化合物6~10为首次从Diaporthe属真菌中分离得到。体外细胞毒活性结果显示化合物4和5对肿瘤细胞株SF-268,MCF-7,NCIH460和Hep G-2具有显著的抑制活性,IC50分别为5.3,6.5,12.2,6.1μmol·L-1和8.2,5.2,6.1,9.4μmol·L-1。
In order to study the active secondary metabolites of the endophytic patchouli endophyte Diaporthe longicolla A616. Ten compounds were isolated from the fermentation product of the strain by normal phase silica gel, C18 reverse phase, Sephadex LH-20 gel and HPLC, and identified by spectral data analysis and literature comparison respectively. (7R, 9R) -7-hydroxy-9-propyl-5-nonen-9-olide (2), ergosta-5,7,22-trien-3β-ol (22E, 24R) -3β, 5α-dihydroxy-6β-ergosta-7,22 (22E, 24R) -ergosta-4,6,8 (14) -22-tetraen- (5) citreoisocoumarin (6), glycerol monolinoleate (7), 1- (2-hydroxyethoxy) ethyl (E) -octadec- 9-enoate (8), cyclo- 9), cyclo (L) -Pro- (L) -Val (10). Among them, compounds 6-10 were isolated from the genus Diaporthe for the first time. In vitro cytotoxic activity of compounds 4 and 5 showed significant inhibitory activity against tumor cell lines SF-268, MCF-7, NCIH460 and Hep G-2 with IC50 of 5.3, 6.5, 12.6 and 6.1 μmol·L -1, respectively 8.2, 5.2, 6.1, 9.4 μmol·L-1.