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目的采用近红外漫反射技术结合偏最小二乘法对维生素C片中的维生素C进行非破坏性定量分析。方法110个校正集样品采用近红外二阶导数在10 000~4 000 cm-1光谱区间建立了维生素C片中维生素C的含量定量分析模型,对验证集进行验证,并计算实际样品的标示含量。结果校正集预测值与真实值的相关系数(r)为0.996 9,校正均方根误差(RMSEC)为1.30。对10个验证集样品进行外部验证,预测均方根误差(RMSEP)为0.656,预测值的平均回收率为97.24%(RSD=4.89%,n=10)。结论本方法简便,快速,准确,适用于药品快速检查和质量控制。
Objective To investigate the non-destructive quantitative analysis of vitamin C in vitamin C tablets by near-infrared diffuse reflectometry combined with partial least squares. Methods A total of 110 calibration samples were used to establish a quantitative analysis model of vitamin C in vitamin C tablets in the spectral range of 10 000-4 000 cm -1 by near-infrared second derivative. The validation set was validated and the actual content of labeled samples . Results The correlation coefficient (r) between the predicted value of the calibration set and the true value was 0.996 9, and the root mean square error of correction (RMSEC) was 1.30. External validation was performed on 10 validation set samples with a root mean square error of prediction (RMSEP) of 0.656 and an average recovery rate of 97.24% (RSD = 4.89%, n = 10). Conclusion This method is simple, rapid and accurate, suitable for rapid inspection and quality control of medicines.