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晚疫病是马铃薯第一大病害。马铃薯抗晚疫病基因R10抗谱广、应用价值大,常用于马铃薯抗病杂交育种。R10位点位于马铃薯11号染色体的短臂末端,与已克隆的抗晚疫病基因R3a同属于抗晚疫病主效位点的单倍型。对包含195个基因型的R10基因的分离群体进行了晚疫病菌株接种和遗传分析,确定R10为抗晚疫病菌株89148-9的主效单基因。应用比较基因组学和BSA分析,开发了6个与R10连锁的分子标记,并将R10定位在1001T和C2_At5g59960两个遗传距离为2.6cM分子标记之间。R10位于已克隆的抗晚疫病基因R3a的近端粒区。本研究所获得的遗传图谱为通过图位克隆和比较基因组学克隆R10基因打下基础。
Late blight is the largest potato disease. Potato resistance to late blight gene R10 broad anti-spectrum, high value, commonly used in potato disease-resistant cross breeding. The R10 locus is located at the short arm end of chromosome 11 of the potato, which belongs to the haplotype of the major locus of resistance to late blight, as well as the cloned resistance to late blight gene R3a. Phytophthora infestans were inoculated and genetic analyzed on a segregating population of R10 gene containing 195 genotypes and R10 was identified as the major single gene of the late blight resistance strain 89148-9. Six molecular markers linked to R10 were developed using comparative genomics and BSA analysis, and R10 was mapped between two markers of genetic distance of 2.6cM at 1001T and C2_At5g59960. R10 is located in the proximal telomeres of the cloned resistance to late blight gene R3a. The genetic map obtained in this study laid the foundation for the cloning of the R10 gene by map-based cloning and comparative genomics.