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目的:研究乙型肝炎转基因小鼠品系C57 TgN(HBVadr2.0)SMMU肝脏的组织学及免疫病理学特征。方法:以168只SPF级乙肝转基因小鼠及15只正常C57BL/6小鼠为研究对象,取肝组织连续切片,常规H E染色,并选取20 例有明显单个核细胞肝内浸润的肝标本,免疫组化原位鉴定白细胞分化抗原的肝内分布。结果:37.5%的转基因小鼠肝脏出现人慢性轻度乙型肝炎样的病理改变,病变率随月龄增大显著增高;32.7%有更轻微的非特异性炎症反应;肝内浸润的单个核细胞多数为CD3+、CD4+细胞,未发现CD57+、CD8+细胞浸润。结论:乙肝转基因小鼠C57 TgN(HBVadr2.0)SMMU肝脏可出现不同程度的病理改变,与人慢性轻度乙肝组织学相类似。
Objective: To study the histological and immunopathological features of C57 TgN (HBVadr2.0) SMMU liver in hepatitis B transgenic mice. Methods: 168 SPF hepatitis B transgenic mice and 15 normal C57BL / 6 mice were used as research objects. Serial sections of liver tissue were stained with HE staining. Twenty liver samples with obvious intrahepatic infiltration of mononuclear cells were selected. Immunohistochemistry in situ identification of intrahepatic distribution of leukocyte differentiation antigen. Results: Chronic mild hepatitis B-like pathological changes appeared in 37.5% of transgenic mice liver with pathological changes significantly increased with age; 32.7% had more mild nonspecific inflammatory reaction; intrahepatic infiltration of mononuclear cells Most of CD3 +, CD4 + cells, found no CD57 +, CD8 + cell infiltration. CONCLUSION: The pathological changes in C57 TgN (HBVadr2.0) SMMU liver of hepatitis B transgenic mice can be observed in different degrees, which is similar to the histology of chronic mild hepatitis B in human.