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本文报道应用倒置相差显微术和显微荧光光度术观察无血清培养条件对NBA_2细胞突起数目和细胞内脂褐素荧光值的影响,以建立神经细胞老化实验研究模型。结果发现:存在着随无血清培养天数的增加,细胞内脂褐素荧光值累积性增加(P<0.01)和培养5天后细胞突起数目渐次性减少(P<0.01)的趋势。结果表明:这一趋势与哺乳类动物和人类老化研究中的神经细胞内脂褐素含量随增龄而增加及神经细胞突起在衰老过程中逐渐减少消失的结果是平行的,因而神经细胞老化实验研究模型建立良好。结果提示:环境的剧烈变化可以改变细胞的老化过程,不良的生存条件可以加速细胞的衰老。
This article reports the use of inverted phase contrast microscopy and microscopic fluorescence spectrophotometry to observe the effects of serum-free culture conditions on the number of neuronal cells and the intracellular lipofuscin fluorescence values of NBA 2 cells to establish an experimental model of neural cell aging. The results showed that there was a trend of decreasing (P <0.01) with the increase of serum-free days, the cumulative increase of intracellular lipofuscin fluorescence (P <0.01) and the number of cell protrusion gradually decreased after 5 days of culture. The results showed that this trend was in parallel with the increase of lipofuscin content in neurons in mammalian and human aging studies with increasing age and the gradual decrease and disappearance of neurite outgrowth in the process of aging. Therefore, the neuronal aging experiment The research model is well established. The results suggest that: the dramatic changes in the environment can change the cell aging process, poor living conditions can accelerate cell aging.