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一、材料与方法1 c myc寡核苷酸的设计、合成:根据c myccDNA序列AUG后的 15个碱基,设计出相应的正义 (SON)、反义(ASON)和错配寡核苷酸。正义 5′ATGCCCCTCAACGTG3′反义 5′CACGTTGAGGGGCAT3′,错配 5′CACGGTGAGGTGCAT3(15对碱基全硫代修饰)。2 大鼠脑胶质瘤模型的建立:
1. Materials and Methods 1 Design and Synthesis of c myc Oligonucleotides: According to the 15 bases after AUG of the c myccDNA sequence, the corresponding sense (SON), antisense (ASON) and mismatched oligonucleotides were designed. . Justice 5’ATGCCCCTCAACGTG3’antisense 5’CACGTTGAGGGGCAT3’, mismatch 5’CACGGTGAGGTGCAT3 (15 bases per-thio modification). 2 Establishment of rat glioma model: