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目的对铁皮石斛化学成分提取并筛选主要活性指标,建立可靠的分离测定方法。方法采用超临界二氧化碳萃取(SFE)和模拟移动床(SMB)对铁皮石斛成分进行提取与分离。结果最优SFE萃取条件下,可得铁皮石斛粗萃物和柚皮素分别为46 g·kg-1和30 mg·kg~(-1);粗萃物中柚皮素在最优超临界流体模拟移动床(SF-SMB)条件下,得率由1 199 mg·kg~(-1)提高至2 400 mg·kg~(-1),粗萃物中特征峰(peak X)含量由4.99%增加至20.44%;分离纯化SF-SMB萃出液中富集的特征峰(peak X),采用GC-MS/IR鉴定出该化合物为4-烯丙基-2,6-二甲氧基苯酚。结论本实验可为铁皮石斛质量控制研究提供良好基础,可为铁皮石斛天然成分的工业化提取提供新思路。
Objective To extract and screen the main chemical activity of Dendrobium officinale and to establish a reliable method of separation and determination. Methods Supercritical carbon dioxide extraction (SFE) and simulated moving bed (SMB) were used to extract and separate the components of Dendrobium candidum. Results Under the optimized conditions of SFE extraction, the extract and naringenin of Dendrobium officinale were 46 g · kg-1 and 30 mg · kg-1, respectively. The concentration of naringenin in the crude extract was the best in supercritical The yield of peak X in crude extracts increased from 1 199 mg · kg -1 to 2400 mg · kg -1 under the condition of SF-SMB, 4.99% to 20.44%. The peak of enrichment in SF-SMB extract was separated and purified. The compound was identified as 4-allyl-2,6-dimethoxy Phenol. Conclusion This experiment can provide a good foundation for the quality control of Dendrobium officinale and provide a new idea for the industrial extraction of the natural components of Dendrobium candidum.