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以穗中红成龄叶片及种子实生苗叶片为材料,通过愈伤组织诱导胚状体和不定芽再生植株。成龄叶片在MS~*+KT1.0mg/L+2,4-D1.0mg/L+3％～4％蔗糖、实生苗叶片在MS~*+kT0.5mg/L+2,4-D1.0mg/L+3％～4％蔗糖或MS~*+2,4-D1.0mg/L+3％～4％蔗糖上培养3～4周均获得质量良好、产量较高的愈伤组织;以MS~*+BA0.5mg/L+NAA0.2mg/L+ADS40mg/L+CH0.5g/L+GA_30.5mg/L+3％蔗糖继代愈伤组织效果较佳,但成龄叶片的愈伤组织出现坏死现象;已获得实生苗叶片胚状体及不定芽,且在一定的培养条件下诱得了植株,成龄叶片仅获得根状物,没有其他结果。本研究旨在为基因工程转化抗病毒基因提供细胞工程方面的转化途径和技术,具有重要的现实意义。
The red leaves and seedling leaves of Suizhong were used as materials to induce embryoid body and adventitious buds to regenerate plants. The leaves of mature aged seedling were MS ~ * + KT1.0mg / L + 2,4-D1.0mg / L + 3% ~ 4% The callus with good quality and high yield was obtained after culturing for 3 ~ 4 weeks on 0.5 mg / L + 3% ~ 4% sucrose or MS ~ * + 2,4-D1.0 mg / L + 3% ~ 4% sucrose ; MS ~ * + BA0.5mg / L + NAA0.2mg / L + ADS40mg / L + CH0.5g / L + GA_30.5mg / L +3% sucrose subculture better callus, The callus of the callus was necrotic. The embryos and adventitious buds of the seedling leaves were obtained, and the plants were induced under certain culture conditions. Only the rooting material was obtained from the aged leaves, with no other results. The purpose of this study is to provide cell-engineering transformation approaches and technologies for genetic engineering of antiviral genes, which is of great practical significance.