论文部分内容阅读
目的 探讨急性哮喘患儿T细胞亚群的变化及意义。方法 采用直接免疫荧光法,应用流式细胞 仪,检测54例急性发作期哮喘患儿及52例健康儿童外周血CD3+T细胞、CD4+T细胞、CD8+T细胞的数值及 CD4+/CD8+比值。结果 与正常对照组比较,急性发作期哮喘患儿外周血中CD4+T细胞显著增高(P<0.05); CD4+/CD8+比较也显著增高(P<0.05);而CD3+T细胞、CD8+T细胞无明显变化(P>0.05)。结论 急性发 作期哮喘患儿外周血中CD4+T细胞、CD4+/CD8+比值均明显高于正常对照组,CD4+T细胞在哮喘慢性气道炎症 中发挥重要作用。
Objective To investigate the changes and significance of T cell subsets in children with acute asthma. Methods The direct immunofluorescence method was used to detect the numbers of CD3 + T cells, CD4 + T cells and CD8 + T cells in peripheral blood and the ratio of CD4 + / CD8 + in 54 acute asthmatic children and 52 healthy children by flow cytometry . Results Compared with the normal control group, CD4 + T cells in peripheral blood were significantly increased (P <0.05) and CD4 + / CD8 + were significantly increased in acute asthmatic children with asthma (P <0.05); while CD3 + T cells, CD8 + T No significant changes in cells (P> 0.05). Conclusion The percentage of CD4 + T cells and CD4 + / CD8 + in peripheral blood of asthmatic children with acute exacerbation is significantly higher than that of normal controls. CD4 + T cells play an important role in chronic airway inflammation in asthma.